Outsourced Pharma App Notes & Case Studies
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Genome Editing of Human Primary T Cells With Lipid Nanoparticles
4/18/2022
We show CRISPR/Cas9-mediated knockouts of the T cell receptor and explore multi-step LNP engineering to produce TCRɑβ KO CAR T cells, a promising approach towards allogeneic CAR T cell therapy.
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Empty/Full Capsid Ratio Of AAV By Sedimentation Velocity – Analytical Ultracentrifugation
9/29/2022
AAV capsids have the potential to impact safety and efficacy. Here we learn more about the results generated from an internal study using AAV8 capsids.
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Manufacturing With A Highly Potent API For An Ophthalmic Indication
1/9/2024
Review this approach to the manufacture of a nano-milled suspension of loteprednol etabonate intermediate, encompassing the entire product development spectrum and repeat client engagement.
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Accelerating Bulk Harvest Testing Using A CHO Animal Origin Free Virus Panel
10/19/2023
Here, we describe the design and performance of a degenerate PCR-based approach to broad virus screening that can be used in testing bulk harvest material derived from CHO cells.
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Illustrating A Successful Small Biotech-CDMO Relationship
4/13/2022
Explore the experience of selecting and working with a global CDMO partner and the value for one virtual company established to develop the molecular design and process development for a complex protein.
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Tangential Flow Filtration System Coupling To Enhance Solution Offerings
3/1/2023
Discover a simple, easy, and versatile method for capsule and depth filtration of small volumes that moves research from cell harvest to purification as quickly and efficiently as possible.
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Enhancing Ease Of Use Batch Turnaround And Process Flexibility With Single-Use TFF
6/17/2022
Use of single-use Pellicon® Capsules is an effective strategy for improving operational flexibility, product safety, and turnaround of a TFF step.
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Lay The Proper Foundation For Upstream Success
4/7/2022
When selecting a partner for development of upstream processes you need speed, quality and regulatory compliance – all appropriately balanced.
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Determining RNA Integrity And Purity By Capillary Gel Electrophoresis
6/30/2023
To ensure mRNA product quality, it's important to confirm the integrity of the RNA. Examine the results of a study challenging the performance of the CGE method in assessing mRNA integrity and purity.
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Recombinant Adeno-Associated Virus Type 5 Production Process
10/12/2022
Learn about a scalable process for the production of rAAV using triple plasmid transfection of suspension cells in an animal-derived component-free medium.