The ability to scale seamlessly between bioreactor sizes helps to ensure product quality and de-risk scaling activities. Bioreactor scaling translates settings from one bioreactor to another. Often, the science is complicated, and it’s challenging to communicate between different teams.
In this article, we present the theory for bioreactor scaling and propose a workflow to scale the agitation and aeration regimes. We highlight the two important boundary conditions: maintaining both the OTR and the volumetric gas flow rate when scaling a process. Furthermore, we describe a simple web-based scaling tool that eases scaling, exploring the design space for agitation and aeration and calculating key parameters that characterize the physical environment in the bioreactor.