Role Of Membrane Chemistry And Filter Format In Tangential Flow Filtration Of mRNA And Lipid Nanoparticles

Ultrafiltration and diafiltration via tangential flow filtration play an important role in multiple stages of RNA and lipid nanoparticle downstream processing. Achieving efficient UF/DF at ambient temperature while preserving product quality requires careful consideration of both membrane chemistry and filter format. A comparison of 100 kDa filters made from polysulfone, polyethersulfone, and regenerated cellulose reveals how performance can vary between hollow fiber and flat sheet cassette configurations. The findings clarify how these variables influence mRNA and LNP process optimization, helping process developers evaluate filtration conditions, manage processing time, and identify suitable approaches for specific applications.

Watch the full analysis to better understand how membrane chemistry and filter format can guide TFF optimization for mRNA and LNP processing.