Robust, Accurate Off-Gas Analyzers Designed For Use In Culture Biosciences High-Throughput Bioreactor Facility

By Helen Luo, Max Gilbert, Dan Chen, Matthew Ball, Will Patrick

Culture Biosciences has developed an off-gas analysis system for bioreactors, a key tool for optimizing bioprocesses. This system integrates off-gas analyzers into each bioreactor for continuous monitoring of metabolic indicators. The system employs three digital sensors to measure O₂ and CO₂ concentrations, temperature, and humidity. These sensors are calibrated every 30 days to maintain accuracy. When tested, the off-gas analysis units demonstrated less than 2% relative drift for O₂ sensors and less than 4% for CO₂ sensors over a 40+ day period. The system was found to have a slightly faster response time than commercially available analyzers. This advancement could significantly improve cultivation processes in bioreactors.