Application Note

rAAV Production Using HEK293 Cell Line And Transfection Medium

Source: Cytiva

By Anna Ucher

Cytiva 4 08A0744

The recombinant adeno-associated virus vector (rAAV), for example, the rAAV5 serotype, has been widely evaluated as a gene therapy delivery system. Here, we show how a HyClone™ peak expression cell culture medium promotes recombinant protein production. We investigated the scalability of the process in HyClone™ peak expression medium in Xcellerex™ XDR-10 and XDR-200 bioreactors, and the performance was compared with the shake flask cultures. HyClone™ peak expression medium showed improved growth, viability, and rAAV productivity in HEK293.2 suspension cells.

Learn more about improving the performance of a scalable AAV process in Xcellerex™ XDR-10 and XDR-200 bioreactors using HyClone™ peak expression medium.

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