Precision at Scale: Optimizing Cell-Free DNA Manufacturing for Next-Generation mRNA Vaccines and Therapeutics

Plasmid templates carry baggage into IVT: bacterial backbone sequences, incomplete linearization, nicked topologies, and polyA tracts that E. coli struggles to hold.

Dr. Ashish Deer, Director of RNA at 4BaseBio, hosted by Myles Skoda of Cell & Gene, presents head-to-head data comparing plasmid with enzymatically produced, backbone-free linear DNA. The comparisons cover RNA yield at reduced template input, dsRNA, capping, and integrity in long and self-amplifying transcripts. He also looks at polyA homogeneity confirmed by orthogonal methods, a personalized cancer vaccine timeline, and how teams can approach comparability when changing a DNA template source. A live Q&A addresses construct length, scale, and analytics.

Watch to hear how the numbers hold up.