Generating A Robust Host Cell Line, GOCHO™, For A Cell Line Development Platform

By Michelle Sabourin, Paula Ravnikar, Zhou Jiang, Nan Lin, and Peggy Lio

A robust cell line development (CLD) platform relies on a host cell line. CLD is notably challenging due to the fact that at least two different media are necessary in order to support suspension growth. Single-cell cloning is required to support low cell density growth, and protein production is needed for high cell density growth. Our goal was to establish a host cell line with the desired growth characteristics and the capability to seamlessly switch between cloning and production media. Learn how cell performance within the context of the cell line development workflow was confirmed with the successful isolation of stable, high-producing clones from three model monoclonal antibodies.