Confident Sialic Acid Identification With MS/MS

A late-stage biologics developer needed to confirm that its glycan profile contained only human-compatible sialic acid before regulatory submission. Traditional mass spectrometry could not distinguish between lookalike molecules with identical masses, leaving uncertainty around the presence of potentially immunogenic species.

Solvias applied MS/MS-based structural analysis to definitively identify the sialic acid species and eliminate ambiguity.

The approach delivered:

• Definitive confirmation of human-compatible NANA through structural analysis
• Exclusion of immunogenic NGNA and other isobaric lookalike species
• Clear, annotated data to strengthen regulatory submissions
• Greater confidence in product quality and patient safety