4BaseBio Capabilities Update September 2026: Viral Vectors

Plasmid backbone sequences can end up packaged inside AAV capsids, a concern that regulators and recent clinical literature have brought into focus. This webinar looks at cell-free synthetic DNA as an alternative template for triple transfection, and at what changes when the DNA is linear and closed at both ends rather than circular.

The speaker, from the cell-free synthetic DNA manufacturer 4basebio (Cambridge, UK), presents transfection optimization data, scale-up results, customer examples using design of experiments, and sequencing analysis of vectors made from each template type. The data raise questions about titer, DNA and transfectant requirements, and residual backbone content.

Watch the webinar to hear how synthetic templates performed against plasmid controls.