Antisense Oligonucleotide Extraction Optimization Reveals Significant Differences Between Non-Human Primate Spinal Cord, Mouse Spinal Cord, And Mouse Brain Tissue Homogenates

This study investigates the most effective techniques for extracting antisense oligonucleotides (ASOs) from spinal cord tissue in both non-human primates and mice, as well as from mouse brain tissue. Given the distinct biochemical and structural properties of these tissues, we demonstrate that a one-size-fits-all approach is insufficient for achieving optimal ASO recovery. Instead, tailored extraction methods are required to maximize yield and maintain the integrity of ASOs across different tissue types.

Our findings provide critical insights into refining ASO extraction protocols, which can enhance the accuracy of downstream analyses and support further research into ASO-based therapeutics.