TARGATT™ HEK293 Kits

DNA insertion simplified

From promoter screening libraries to protein expression and stable viral vector production, TARGATT™ HEK293 Kits put efficient and precise cell line development into your hands.

Leveraging the power of site-specific integration and the consistently-expressing H11 safe harbor site, our TARGATT™ large knock-in technology enables genome engineering that CRISPR, transposons, and lentivirus can’t handle.

Expand what you can accomplish in HEK293 cells with TARGATT™ technology

Available TARGATT™ HEK293 Kits

TARGATT™ Kits are designed to enable easy evaluation of the technology before purchasing a license*. Each TARGATT™ HEK293 Kit includes a HEK293 cell line with the TARGATT™ landing pad already inserted into the H11 safe harbor site, a donor plasmid for the DNA you are knocking in, a positive control plasmid, and a TARGATT™ Integrase expression plasmid.

*Academic researchers may purchase TARGATT™ technology without licensing. Request more information.

• Kits are available as a general purpose kit for protein/multi-protein expressio (Cat.# AST-1305) or pre-configured for library creation with either drug selection (Cat.# AST-1306) or FACS (Cat.# AST-1308).
• We also offer additional plasmids that enable inducible expression. Learn more by downloading our poster and then contact us and specify whether you wish to try approach A or C.
• If you need additional kit components, please contact us.

Why you should choose TARGATT™ HEK293 Kits

• Efficient integration—over 90% after selection
• Consistent expression from clone to clone, enabling the use of pools instead of clonal purification
• Large cargo knock-in—up to 20 kb in a single reaction, more with nested reactions
• Site-specific integration into the H11 safe harbor site
• Single-copy insertion

Learn how TARGATT™ technology works and see the data—visit the technology page.

Because integration using TARGATT™ technology is so efficient, you can create highly diverse libraries and even perform mammalian display for screening in a biologically-relevant cell type.

^{Publications about TARGATT™ technology}

• ^{Chi, X., Zheng, Q., Jiang, R., Chen-Tsai, R. Y., & Kong, L. J. (2019). A system for site-specific integration of transgenes in mammalian cells. PLOS ONE, 14(7), e0219842.}

• ^{Zhu, F., Gamboa, M., Farruggio, A. P., Hippenmeyer, S., Tasic, B., Schüle, B., … Calos, M. P. (2014). DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Nucleic Acids Research, 42(5), e34. http://doi.org/10.1093/nar/gkt1290.}