Streamlined Pre-Formulation Screening With Minimal Sample Requirements & 2-Day Turnaround To Enable High Efficiency AAV Formulation Development

By Philipp Beck, Astrid Stockinger, and Sonya M. Schermann

This study investigates the effects of pH and sodium chloride (NaCl) concentrations on particle size, dispersity, and protein stability in rebuffered solutions, using dynamic light scattering (DLS) measurements. The analysis focuses on particles larger than 2 µm, 10 µm, and 25 µm under varying pH levels (5 to 8) and NaCl concentrations. Aggregation temperatures were found to vary with changes in pH and NaCl, while capsid melting temperatures exhibited distinct profiles under different conditions.

Additionally, recovery rates post-rebuffering were evaluated, highlighting the efficiency of the process across the tested parameters. The findings offer valuable insights into optimizing conditions for protein stability and performance, providing important implications for biotechnological applications.