Strategies For Engineering Mammalian Cells

Engineering transgenic mammalian cell lines for protein production is often complex and time-consuming. Traditional methods like CRISPR/Cas9 struggle with large DNA insertions, while alternatives such as lentivirus and piggyBac transposons integrate randomly, requiring extensive screening for optimal expression. This challenge is further amplified when producing cytotoxic proteins, complicating stable expression and scalability.

To overcome these hurdles, we compare strategies for generating both clonal and pooled expression lines. Using cells with a pre-engineered TARGATT™ landing pad, we achieve inducible expression within just 4-6 weeks after donor plasmid creation. This streamlined approach significantly reduces development time and effort, offering a scalable and efficient solution for protein production in research and biopharmaceutical applications.