Rapid AAV Pre-Formulation Screening Using A Commercially Available Buffer Kit & Minimal Sample Requirement

By Philipp Beck, Astrid Stockinger, and Sonya Schermann

This poster presents a rapid AAV pre-formulation screening method using a commercially available buffer kit with minimal sample requirements. The study tested 96 different formulations to assess capsid and colloidal stability under varying conditions of pH, temperature, and ionic strength. Utilizing nanoDSF and DLS techniques, the screening evaluated the thermal stability and aggregation behavior of AAV samples with as little as 10–40 µL per condition. The results demonstrate the impact of different buffer compositions, including acetate, citrate, histidine, and PBS, with and without NaCl, on AAV stability.

By systematically identifying optimal formulations, this approach enables efficient and high-throughput selection of stable AAV formulations, reducing development time and material consumption. The findings support the implementation of streamlined pre-formulation screening to enhance AAV vector stability for gene therapy applications.