Mastering Critical Binding Reagents In Immunoassays: The Key To Precision, Consistency, And Success

Dr. Tom Zhang, Chief Scientific Officer, Large Molecule Bioanalysis, Dr. Andrew Cunningham, Vice President, Laboratory

Immunoassays are pivotal tools in the detection and quantification of biomolecules. The critical binding reagents—capture and detection antibodies—are central to the precision and reliability of these assays, driving the specificity and sensitivity of the results. The quality of these reagents directly influences the outcomes of drug discovery, development, and clinical trials. However, the intricacies of selecting, generating, and characterizing these reagents require a deep understanding of both the science and the practical challenges involved.

In this white paper, you will delve into the critical role of binding reagents in immunoassays, exploring the key considerations for their selection, the detailed processes involved in their generation, and the rigorous methods used for their characterization. By examining the nuances of monoclonal and polyclonal antibody production, as well as the various labeling techniques, you will uncover how these factors contribute to assay accuracy and consistency. Furthermore, it discusses best practices for reagent management, ensuring that the integrity and performance of assays are maintained throughout the drug development process. Download the white paper below to unlock insights into optimizing immunoassay workflows to achieve reliable, reproducible results.