How To Maximize Cell Line Productivity And Efficiency

In this Q&A, Dr. Tabitha Bullock, a principal group leader at Lonza, provides insights into key considerations in cell line development and how advancements in technology are improving efficiency and productivity in this field.

Explore the importance of selecting a host cell line with proper authentication documentation to ensure the reliability and traceability. Additionally, choosing the right transfection method and vector is crucial for successful cell line development.

Enhancing protein expression is another important aspect of cell line development. Selecting the right expression system technology and incorporating insulator elements and 3'UTR sequences can improve protein expression.

Recent advancements in technology have further improved cell line development. Transposon-based cell line engineering, high-throughput omics technologies, gene editing, and automation have all contributed to increased efficiency and productivity in this field.

CDMOs are expected to adopt a shift towards single-cell analysis in cell line development, which involves measuring the productivity of individual cells rather than analyzing heterogeneous populations. This may impact stability, and high-throughput screening tools need to be established to support this approach.