Flavivirus vaccine development and production constitute many challenges and can be both space- and resource-consuming. This white paper gives an overview of modern tools and solutions, adding flexibility and speed to both upstream and downstream operations in flavivirus vaccine production. Single-use production bioreactors and chromatography purification columns mitigate cross-contamination risk and support increased operator safety, while reducing time to market by eliminating costly and time-consuming cleaning operations. By allowing for quick startup and changeover between productions, single-use technologies provide the flexibility required to quickly adapt to market needs. For downstream processing, modern chromatography resins offer high selectivity and excellent pressure-flow properties for high productivity in manufacturing-scale purifications.