TRiP System™ For AAV, Lentiviral Vectors And Adenoviral Vectors

Our ‘Transgene Repression in Vector production’ (TRiP) System™ temporarily stops the production of transgene protein during manufacture of vector particles in order to increase vector production. The TRiP System™ can potentially improve vector yield by up to 1000 times, thereby decreasing the cost of goods for manufacturing as well as increasing quality. It also enables the manufacturing of vectors carrying cytotoxic transgenes, or those that inhibit cell growth. The TRiP System™ has no impact on the expression of the transgene in target cells.

The TRiP System™ works by transfecting a plasmid encoding the bacterial tryptophan RNAbinding attenuation protein (TRAP) in production cells. A TRAP binding sequence (tbs) is inserted upstream of the transgene in the vector genome. In production cells, in excess of L-tryptophan, the TRAP protein binds to the tbs sequence and blocks translation of the transgene mRNA.