Download | February 23, 2007

Technical Bulletin: T040 Protein Purification Techniques, Volume 1, Ionic Precipitation

Source: SAFC
Protein precipitation achieves separation by the conversion of soluble proteins to an insoluble state, which subsequently can be removed by various means. Precipitation can be used to remove components in cell culture media that may interfere with downstream purification methods. Ideally, precipitation results in both concentration and purification. Thus, precipitation is often used early in the sequence of downstream purification, reducing the volume and increasing the purity of the protein prior to any chromatography steps.

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