SecNuc™ For AAV, Lentiviral Vectors And Adenoviral Vectors

Residual DNA is a common problem in vector production caused by plasmid DNA remaining after transfection and by host cell DNA resulting from cell death after the release of the virions.

The current industry standard approach is to reduce residual DNA level by using a GMP-grade recombinant nuclease, such as Benzonase^®, to treat harvested vector material prior to downstream purification. However, this treatment is expensive when considering large-scale manufacturing and its effectiveness can be limited.

Our SecNuc™ Solution

We have developed a highly efficient alternative approach to bypass the Benzonase^® step by using secreted nucleases in co-production with viral vector manufacture.