Mass Propagation Of Nicotiana Tabacum Cv. BY‐2 Suspension Cells In The Hyperforma Rocker Bioreactor

By Nadezda Perepelitsa, GMP Process Engineer, Johnson & Johnson, Basel, Switzerland; Stephan C. Kaiser, Staff Scientist, Research and Development, Thermo Fisher Scientific; Dieter Eibl, Professor, ZHAW School of Life Sciences and Facility Management, Centre for Biochemical Engineering and Cell Cultivation Technique, Wädenswil, Switzerland; Nicole Imseng, Process Expert USP/DSP, CSL Behring, Lengnau, Switzerland

This application note describes the mass propagation of Nicotiana tabacum cv. BY‐2 (cultivar tobacco Bright Yellow‐2) suspension cells in the Thermo Scientific™ HyPerforma™ Rocker Bioreactor at laboratory scale. Maximum fresh cell weights of 351 ± 9 g/L, corresponding to packed cell volumes of 74% ± 2%, were achieved, which shows the ability of the HyPerforma Rocker Bioreactor to cultivate fast-growing plant suspension cells.