Improving Fed-Batch Yields By Combining EX-CELL® Advanced And Cellvento® Cell Culture Media Portfolios

By Jonathan Kaiser, Senior Scientist, M. Sc., Innovation Cell Culture Media Development, BioProcessing R&D and Melanie Brandl, Ph.D., Global Product Manager Proprietary Media

The biopharmaceutical industry is interested in developing high-titer fed-batch processes to meet increasing market demands and reduce manufacturing costs. While these processes can achieve increased cell densities which support improved volumetric productivity, they also have high nutrient demands. Nutrient limitations can be prevented with addition of a highly concentrated feed at different time points during the culture to help sustain the production phase. Given the diversity of media platforms, Chinese hamster ovary (CHO) cell characteristics and process settings, establishing a cost- and time-efficient approach for process optimization is essential. This application note provides guidance and options for improving culture yield with off-the-shelf catalog products. Media combinations, dynamic fed-batch feeding options, and feed screening strategies suitable for diverse cell lines and manufacturing scenarios are presented.