Development Of An Intensified Fed-Batch Process Utilizing N-1 Perfusion And ambr®15
While traditional fed-batch bioreactor process development has been well defined using methods such as shake flasks and bench-scale bioreactors, there is a desire to intensify the fed-batch process strategy to increase efficiency and productivity. Utilization of perfusion during the N-1 stage allows for inoculation of the production bioreactor at a very high cell density. Learn how to combine N-1 perfusion with the ambr®15 bioreactor system to create a powerful tool for rapid development of a highly efficient cell culture process.
- How N-1 perfusion can increase product titer and/or decrease production time
- How ambr®15 can be utilized to rapidly screen a variety of media conditions using a DOE approach
- How N-1 perfusion and ambr®15 can be utilized together to quickly identify top conditions to scale up to 2L bioreactor
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