Application Note

Cultivation Of Sf-9 Insect Cells And SEAP Expression In The Finesse «SmartGlass» Bioreactor

Source: Thermo Fisher Scientific
Products_Vessels_SmartRocker_Header2

This application note describes the cultivation of Sf-9 suspension cells in the Finesse «SmartGlass» vessel bioreactor having a maximum working volume of 2.0 L. Using serum-free medium without components of animal origin viable cell densities of up to 4.964∙106 cells∙mL-1 were achieved. The SEAP expression was induced by the baculovirus expression vector system, which provided maximum SEAP activities of 56.4 U∙mL-1. By Stephan C. Kaiser, Ina Dittler, Nina Steiger and Dieter Eibl

VIEW THE APPLICATION NOTE!
Signing up provides unlimited access to:
Signing up provides unlimited access to:
  • Trend and Leadership Articles
  • Case Studies
  • Extensive Product Database
  • Premium Content
HELLO. PLEASE LOG IN. X

Not yet a member of Outsourced Pharma? Register today.

ACCOUNT SIGN UP X
Please fill in your account details
Login Information
I'm interested in newsletter subscriptions.
ACCOUNT SIGN UP

Subscriptions

Sign up for the newsletter that brings you the industry's latest news, technologies, trends and products.

You might also want to: