This application note describes monoclonal antibody (MAb) purification processes using the expanded MAb purification toolbox of GE Healthcare’s Life Sciences business, covering modern chromatography media (resins) for standard as well as more challenging purification tasks. The most established approach for purifying MAbs is a three-step process, where the initial capture step using a protein A medium is followed by two polishing steps using cation exchange (CIEX) and anion exchange (AIEX) media. Typical results from such an approach will be presented. However, for more challenging MAb purifications, an expanded MAb purification toolbox is beneficial. A study, in which a multimodal Capto adhere ImpRes AIEX medium was used as an alternative in the final purification step, will be presented. Using this second approach, the set criteria for the polishing steps were achieved, with a recovery of above 90% over each step and an aggregate content below 1% in the final product.